![]() ![]() The Fusion offers two- and four-way bulk sorting devices for 1.5mL microfuge tubes, 12 x 75mm 5mL tubes or 15mL conical tubes. Three sizes of nozzles (70, 85 and 100-micron) are offered to accomodate a wide range of particle sizes. The Fusion has been verified to meet personnel and product protection standards for a Class II, Type A2 biosafety cabinet as specified by the National Sanitation Foundation International Standard 49. The Fusion sits inside a custom designed biosafety cabinet to address the concerns of biological safety issues and to protect operators from potential accidental exposure from biological samples. The BD Fusion is a state-of-the-art cell sorter equipped with five dedicated lasers (355nm, 405nm, 488nm, 561nm, and 637nm) for excitation and 20 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 18 fluorescence parameters. To schedule assisted use or training please contact Richard Salinas.įees: trained user: $45/hour assisted use: $115/hour Instrument Access: Assisted use and training are available. Instrument Configuration: Fortessa Config Fluorescence Spectrum Analyzer (BD Biosciences) The Fortessa only accepts 5mL polystyrene tubes, or 96- and 384-multi-well plates if using the HTS unit. Sample analysis can also be done using Flowjo or FCS Express (both available to use in NMS2.114). Sample acquisition and analysis is done using FACSDiva v6.1.3. In addition, this system is also equipped with the FACSFlow Supply System to allow larger volume tanks for sheath and waste, as well as improved fluidic stability. This instrument can be connected to the optional High Throughput Sampler (HTS) unit to accommodate automated specimen handling for 96- and 384-well plates. The Fortessa is equipped with four lasers (405nm, 488nm, 561nm and 635nm) and 15 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 13 fluorescence parameters.
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